Axis BTA 2100D Manuel de l'opérateur télécharger pdf (Page 217)

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Customize Parameters window is separated into three sections. Ionization,

Isolation and Dissociation.

Ionization

Ionization Storage Level: The m/z value at which the rf is maintained during

ionization and the coarse isolation step. The range is 35 to 160 m/z, with a

default value of 48 m/z.

Ejection Amplitude: the amplitude (in volts) of the ejection waveform during the

coarse isolation step. The default value will work for most ions. For certain ions

that are less stable, this value may need to be reduced in order to minimize the

amount of energy imparted to the parent ion during isolation. The range of the

ejection amplitude is from 0 to 60 volts, with a default value of 20 volts.

Isolation

Low Edge Offset: The integer value step to optimize the ejection of the mass

just below the parent ion mass. The low edge offset range is 0 to 20 steps. The

default value is 6 .

Low Edge Offset affects the isolation window on the low mass side of the parent

ion. Increasing the value in the positive direction (increasing the default from 6 to

7 steps) opens the isolation window on the low mass side of the parent ion.

Decreasing the offset (decreasing from the default 6 to 5 steps) decreases the

window on the low mass side. The offset should be adjusted to minimize the

amplitude of the adjacent masses below the parent ion. Initially, adjust in 2 step

increments. Typically, a 2 step adjustment will eliminate the mass below the

parent ion.

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Axis BTA 2100D Manuel de l'opérateur Page 217

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